UHPLC Column, RP PFP, 1.8um, 100A. 2.1mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68122‑05P‑2 Cogent RP PFP™ UHPLC Column is designed for enhanced selectivity of halogenated, aromatic, and polar compounds, delivering improved resolution where traditional C18 phases may lack specificity.
This stationary phase features a pentafluorophenyl (PFP) ligand bonded via a 4‑carbon linker to high‑purity silica with full double end‑capping, providing multiple interaction mechanisms including π‑π interactions, dipole interactions, and hydrogen bonding. This results in greater selectivity control and improved separation of structurally similar analytes.
The phase is particularly effective for USP L43 methods, as well as new method development where alternative selectivity is required.
50 mm Length – Fast UHPLC Screening & Optimization
The 50 mm length is optimized for rapid separations and high‑throughput method development, especially in LC‑MS workflows.
Short run times and rapid gradients
Efficient selectivity screening
Reduced solvent consumption
✅ Use when speed and rapid optimization are priorities
Flow Rate & System Requirements (2.1 mm ID, 1.8 µm)
⚠️ Requires UHPLC system capability due to higher backpressure
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.25–0.35 mL/min
Requires precise, stable low‑flow pumping
Compatible with low‑volume LC‑MS detectors
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with fluorinated and halogenated analytes
Improved resolution compared to C18
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater selectivity flexibility in method development
Improved Resolution Without Increasing Run Time
Maintains resolution while enabling faster methods
Useful for optimizing existing methods
Cogent RP PFP™ Advantages
Fully End‑Capped Surface
Minimizes secondary silanol interactions
Improves peak symmetry for polar compounds
High Efficiency (1.8 µm Particles)
High plate counts and peak capacity
Faster separations with maintained resolution
High Purity Silica
Consistent performance and reproducibility
Reliable method transfer and validation
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Pharmaceutical method development
LC‑MS analytical workflows
USP L43 method applications
Important Usage Notes
⚠️ Requires UHPLC system (high pressure capable)
Designed for low‑flow LC‑MS and UHPLC workflows
Higher backpressure than larger particle size columns
Use 0.2 µm filtration to maintain column performance